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Image Search Results
Journal: Oncotarget
Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?
doi: 10.18632/oncotarget.27185
Figure Lengend Snippet: ( A ) PEDF mRNA expression in ovarian control or cancer cells. *** p < 0.005 ( B ). PEDF protein level in serum of control, ovarian cancer patients, and in the acellular fraction of ascites obtained from ovarian cancer patients. * p < 0.05.
Article Snippet: PEDF protein was quantified using a
Techniques: Expressing, Control
Journal: Oncotarget
Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?
doi: 10.18632/oncotarget.27185
Figure Lengend Snippet: ( A ) Dose-response effect of recombinant PEDF (rPEDF) on SKOV3 cell viability. The 50% inhibitory concentration (IC50) of recombinant PEDF was calculated using Compusyn software.( B ) Determination of the minimal rPEDF concentration allowing additional effect with paclitaxel (Ptx at 100 nM, for 48h) on SKOV3 cell viability.
Article Snippet: PEDF protein was quantified using a
Techniques: Recombinant, Concentration Assay, Software
Journal: Oncotarget
Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?
doi: 10.18632/oncotarget.27185
Figure Lengend Snippet: ( A ) Anti-PEDF antibodies (light grey bars) or control IgG (dark grey bars) were added in culture medium of cells treated with 100 nM Ptx. FBS: fetal bovine serum. ( B ) Western blot analysis of PEDF in FBS and ascites ( C ) Western blot analysis of PEDF in ascites, PEDF-depleted ascites (A-PEDF) and purified PEDF (pPEDF). Left panel: Ponceau red staining of membrane; Right panel: PEDF immunoblotting. ( D ) Effect of ascites-PEDF on viability of cells treated with 100 nM Ptx (for 48h) compared to FBS-PEDF. ( E ) Effect of PEDF depletion from ascites on Ptx treated SKOV3-derived tumor growth. ** p < 0.01.
Article Snippet: PEDF protein was quantified using a
Techniques: Control, Western Blot, Purification, Staining, Membrane, Derivative Assay
Journal: Oncotarget
Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?
doi: 10.18632/oncotarget.27185
Figure Lengend Snippet: ( A ) Evaluation of Ptx treatment in combination with ascites, PEDF-depleted ascites (ascites-PEDF) or purified PEDF (pPEDF) treatment on COV318-derived tumor growth. ( B ) Evaluation of recombinant PEDF (rPEDF) or purified PEDF (pPEDF) treatment on COV318-derived tumor growth. * p < 0.05 and ** p < 0.01.
Article Snippet: PEDF protein was quantified using a
Techniques: Purification, Derivative Assay, Recombinant
Journal: Oncotarget
Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?
doi: 10.18632/oncotarget.27185
Figure Lengend Snippet: ( A ) GFP staining of SKOV3 cells stably transfected with PEDF or control transposon at passage 52 (P52). Magnification: ×200. ( B ) PEDF mRNA level in SKOV3 cells stably transfected with PEDF transposon at different passages (P). ( C ) Time-course of PEDF secretion in culture medium of SKOV3 cells stably transfected with PEDF transposon (grey) or control transposon (black) during 96 h of culture.
Article Snippet: PEDF protein was quantified using a
Techniques: Staining, Stable Transfection, Transfection, Control
Journal: Oncotarget
Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?
doi: 10.18632/oncotarget.27185
Figure Lengend Snippet: ( A ) Growth kinetic of PEDF-SKOV3 (grey) or CTRL-SKOV3-derived tumor (black). ( B ) Photographs (upper panel; magnification ×16.5) and Hemalun Eosin staining (middle panel; magnification ×100) of CTRL-SKOV3 and PEDF-SKOV3-derived tumors at EDD13.5. Enlargement of the black squares was presented in the lower panels (magnification ×400). Tumors are indicated by black arrows, and CAM and tumor vessels by white arrows.
Article Snippet: PEDF protein was quantified using a
Techniques: Derivative Assay, Staining